Summary: Off-target binding and cleavage by Cas9 pose major challenges in its application.How the conformational dynamics of Cas9 govern its nuclease activity under on- and off-target conditions remains largely unknown.Here, using intra-molecular single-molecule fluorescence resonance energy transfer measurements, we revealed that Cas9 in apo, sgRN
Coordinated Design of Power System Stabilizer and Virtual Inertia Control Using Modified Harris Hawk Optimization for Improving Power System Stability
In the current era, power system stability faces typical problems due to the Renewable Energy Sources (RES) integration trend.This trend makes the coordination between power system controllers crucial to maintain stability across a wide-range of operating behaviors.To address this problem, this paper proposes the coordinated design of Power System
PROBLEM-BASED LEARNING METHOD USE IN THE CIVIL CONSTRUCTION ORGANIZATIONAL ENVIRONMENT
The changes in the civil construction context, and consequently the production management of constructions, require a different profile engineer, which demands more refined skills from those professionals, Round Counter Height Table such as innovation, focus on customer, production planning and control, quality management systems knowledge, sustain
Genome-wide identification and characterization of the bHLH gene family and analysis of their potential relevance to chlorophyll metabolism in Raphanus sativus L
Abstract Background Green-fleshed radish (Raphanus sativus L.) is an economically important root vegetable of the Brassicaceae family, and chlorophyll accumulates in its root tissues.It was reported that the basic helix-loop-helix (bHLH) transcription factors play vital roles in the process of chlorophyll metabolism.Nevertheless, a comprehensive st